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Image Search Results
Journal: Arteriosclerosis, Thrombosis, and Vascular Biology
Article Title: PAR2 (Protease-Activated Receptor 2) Deficiency Attenuates Atherosclerosis in Mice
doi: 10.1161/atvbaha.117.310082
Figure Lengend Snippet: Figure 1. PAR2 (protease-activated receptor 2) mRNA and protein are increased in the atherosclerotic prone regions of mice and humans. Normal mouse aortic arch (low-density lipoprotein receptor–deficient [Ldlr−/−] mice fed a chow diet for 24 wk) or atherosclerosis-containing aortic arches (Ldlr−/− mice fed a Western diet for 24 wk) were examined for (A) mRNA extrapolated to normal and (B) protein expression (n=5 individual samples per group). Normal or diseased human carotid arteries were isolated and examined for (C) mRNA extrapolated to normal and (D) protein expression (n=5 individual samples per group). Immunologic detection of PAR2 expression was also performed on mouse atherosclerotic aortic sinus (E, F) and human carotid atherosclerotic lesions (H, I; n=4–5 individual samples per species; ×4 magnification E and H; ×10 magnification F and I). G, J, Quantification of PAR2 staining in the lesion or media of atherosclerotic lesions. Solid white box is zoomed in area. Dashed white line is the area of the lesion. Histobars represent mean±SEM. *P<0.05 for comparisons to normal aorta/artery or lesion vs tunica media (2-tailed Student t test).
Article Snippet: Immunostaining was performed on frozen serial sections as described previously.26
Techniques: Western Blot, Expressing, Isolation, Staining
Journal: Arteriosclerosis, Thrombosis, and Vascular Biology
Article Title: PAR2 (Protease-Activated Receptor 2) Deficiency Attenuates Atherosclerosis in Mice
doi: 10.1161/atvbaha.117.310082
Figure Lengend Snippet: Figure 1. PAR2 (protease-activated receptor 2) mRNA and protein are increased in the atherosclerotic prone regions of mice and humans. Normal mouse aortic arch (low-density lipoprotein receptor–deficient [Ldlr−/−] mice fed a chow diet for 24 wk) or atherosclerosis-containing aortic arches (Ldlr−/− mice fed a Western diet for 24 wk) were examined for (A) mRNA extrapolated to normal and (B) protein expression (n=5 individual samples per group). Normal or diseased human carotid arteries were isolated and examined for (C) mRNA extrapolated to normal and (D) protein expression (n=5 individual samples per group). Immunologic detection of PAR2 expression was also performed on mouse atherosclerotic aortic sinus (E, F) and human carotid atherosclerotic lesions (H, I; n=4–5 individual samples per species; ×4 magnification E and H; ×10 magnification F and I). G, J, Quantification of PAR2 staining in the lesion or media of atherosclerotic lesions. Solid white box is zoomed in area. Dashed white line is the area of the lesion. Histobars represent mean±SEM. *P<0.05 for comparisons to normal aorta/artery or lesion vs tunica media (2-tailed Student t test).
Article Snippet:
Techniques: Western Blot, Expressing, Isolation, Staining