Journal: Medical Devices (Auckland, N.Z.)

Article Title: Consistency and sealing of advanced bipolar tissue sealers

doi: 10.2147/MDER.S79642

Figure Lengend Snippet: Ex vivo burst pressures for porcine carotid arteries transected with ENSEAL G2 and LigaSure. Notes: Median values (mmHg) and total number of vessels included (n), are shown. + P <0.05 comparing LigaSure with both ENSEAL devices; *outlier data point.

Article Snippet: Fresh Porcine carotid arteries were obtained from Animal Biotech Industries (Danboro, PA, USA).

Techniques: Ex Vivo

Journal: Free radical biology & medicine

Article Title: Long-Term Effect of PROLI/NO on the Proliferative and Phenotypic Kinetic Profile after Arterial Injury

doi: 10.1016/j.freeradbiomed.2015.11.027

Figure Lengend Snippet: The carotid artery balloon injury model was performed in 10-week-old Sprague Dawley rats with or without periadventitial application of PROLI/NO (10 mg), or proline (5.5 mg). Carotid arteries were harvested at 2 weeks, 8 weeks and 6 months. A) Representative hematoxylin & eosin stained rat carotid artery sections from each treatment group (10X objective and 20X objective). B) Quantification of the intima to media (I/M) area ratio, and C) percent occlusion shows that PROLI/NO provided durable inhibition of neointimal development at all time points evaluated (N= 4 for proline, N=10 for injury alone, N=7 for the rest of the groups, *P<0.05 vs injury, #P<0.05 vs proline).

Article Snippet: Whereas PROLI/NO inhibits proliferation and cellularity in the intima and media at all time points without having an effect on cell death, it initially inhibits but then stimulates proliferation and cellularity in the adventitia. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 3 caption a7 caption a8 PROLI/NO differentially affects cellularity throughout the arterial wall after balloon injury The carotid artery balloon injury model was performed in 10-week-old Sprague Dawley rats with or without periadventitial application of PROLI/NO (10 mg).

Techniques: Staining, Inhibition

Journal: Free radical biology & medicine

Article Title: Long-Term Effect of PROLI/NO on the Proliferative and Phenotypic Kinetic Profile after Arterial Injury

doi: 10.1016/j.freeradbiomed.2015.11.027

Figure Lengend Snippet: The carotid artery balloon injury model was performed in 10-week-old Sprague Dawley rats with or without periadventitial application of PROLI/NO (10 mg). Carotid arteries were harvested at different time points. BrdU (100 mg/kg, intraperitoneal) was administered 24 hours and 1 hour prior to sacrifice; BrdU-positive nuclei were counted in four high power fields per section and averaged. A) Total cell count of BrdU-positive cells throughout the arterial wall. B) BrdU-positive cell count in the intima. C) BrdU-positive cell count in the media. D) BrdU-positive cell count in the adventitia. E) Representative arterial cross sections 2 weeks after balloon injury. The black arrow heads indicate representative BrdU-positive cells, and the blue arrow heads point to representative BrdU-negative cells (only a few representative cells are labeled). #P<0.05 vs. uninjured control, *P<0.05 vs. injury alone, N=7/treatment group.

Article Snippet: Whereas PROLI/NO inhibits proliferation and cellularity in the intima and media at all time points without having an effect on cell death, it initially inhibits but then stimulates proliferation and cellularity in the adventitia. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 3 caption a7 caption a8 PROLI/NO differentially affects cellularity throughout the arterial wall after balloon injury The carotid artery balloon injury model was performed in 10-week-old Sprague Dawley rats with or without periadventitial application of PROLI/NO (10 mg).

Techniques: Cell Counting, Labeling, Control

Journal: Free radical biology & medicine

Article Title: Long-Term Effect of PROLI/NO on the Proliferative and Phenotypic Kinetic Profile after Arterial Injury

doi: 10.1016/j.freeradbiomed.2015.11.027

Figure Lengend Snippet: The carotid artery balloon injury model was performed in 10-week-old Sprague Dawley rats with or without periadventitial application of PROLI/NO (10 mg). Carotid arteries were harvested at different time points and nuclei were counted in four high power fields per section and averaged. A) Total cell count throughout the arterial wall. B) Cell count in the intima. C) Cell count in the media. D) Cell count in the adventitia. #P<0.05 vs. uninjured control, *P<0.05 vs. injury alone, N=7/treatment group.

Article Snippet: Whereas PROLI/NO inhibits proliferation and cellularity in the intima and media at all time points without having an effect on cell death, it initially inhibits but then stimulates proliferation and cellularity in the adventitia. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 3 caption a7 caption a8 PROLI/NO differentially affects cellularity throughout the arterial wall after balloon injury The carotid artery balloon injury model was performed in 10-week-old Sprague Dawley rats with or without periadventitial application of PROLI/NO (10 mg).

Techniques: Cell Counting, Control

Journal: Free radical biology & medicine

Article Title: Long-Term Effect of PROLI/NO on the Proliferative and Phenotypic Kinetic Profile after Arterial Injury

doi: 10.1016/j.freeradbiomed.2015.11.027

Figure Lengend Snippet: The carotid artery balloon injury model was performed in 10-week-old Sprague Dawley rats with or without periadventitial application of PROLI/NO (10 mg). Mean arterial blood pressure (MAP) and heart rate were measured before anesthetizing the rats. Blood was collected from the tail vein before and 2 hours after surgery. After the post-surgery blood draw, rats were allowed to recover from anesthesia and the MAP and heart rate measured again. Nitrite was measured in plasma by the tri-iodide method. cGMP levels were assessed with a colorimetric kit. A) Plasma nitrite. N=5/treatment group, *P<0.05; B) MAP. N=5, P=NS; C) Heart rate. N=4, P=NS; and D) Plasma cGMP concentration. N=5, P=NS.

Article Snippet: Whereas PROLI/NO inhibits proliferation and cellularity in the intima and media at all time points without having an effect on cell death, it initially inhibits but then stimulates proliferation and cellularity in the adventitia. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 3 caption a7 caption a8 PROLI/NO differentially affects cellularity throughout the arterial wall after balloon injury The carotid artery balloon injury model was performed in 10-week-old Sprague Dawley rats with or without periadventitial application of PROLI/NO (10 mg).

Techniques: Clinical Proteomics, Concentration Assay

Journal: Free radical biology & medicine

Article Title: Long-Term Effect of PROLI/NO on the Proliferative and Phenotypic Kinetic Profile after Arterial Injury

doi: 10.1016/j.freeradbiomed.2015.11.027

Figure Lengend Snippet: The carotid artery balloon injury model was performed in 10-week-old Sprague Dawley rats with or without periadventitial application of PROLI/NO (10 mg). Carotids were harvested at different time points. Evans blue (0.5 ml 0.5%) was administered intravenously immediately before sacrifice. Blue stained area and total injured area was quantified using ImageJ (N=3/treatment group). A) Representative gross images of the harvested carotid arteries showing the Evans blue stain. B) Quantification of the blue area (i.e., denuded endothelium) divided by total injured area, represented as percent.

Article Snippet: Whereas PROLI/NO inhibits proliferation and cellularity in the intima and media at all time points without having an effect on cell death, it initially inhibits but then stimulates proliferation and cellularity in the adventitia. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 3 caption a7 caption a8 PROLI/NO differentially affects cellularity throughout the arterial wall after balloon injury The carotid artery balloon injury model was performed in 10-week-old Sprague Dawley rats with or without periadventitial application of PROLI/NO (10 mg).

Techniques: Staining

Journal: Free radical biology & medicine

Article Title: Long-Term Effect of PROLI/NO on the Proliferative and Phenotypic Kinetic Profile after Arterial Injury

doi: 10.1016/j.freeradbiomed.2015.11.027

Figure Lengend Snippet: The carotid artery balloon injury model was performed in 10-week-old Sprague Dawley rats with or without periadventitial application of PROLI/NO (10 mg). Carotid arteries were harvested at different time points and sections were stained with an antibody to α-SMA. Quantification was performed by grading on a scale of 0–4 by a blinded investigator. A) Total grade sum throughout the arterial wall. B) Grade for the intima. C) Grade for the media. D) Grade for the adventitia. E) Representative arterial cross sections 2 weeks after balloon injury. #P<0.05 vs. uninjured control, *P<0.05 vs injury alone, N=7/treatment group.

Article Snippet: Whereas PROLI/NO inhibits proliferation and cellularity in the intima and media at all time points without having an effect on cell death, it initially inhibits but then stimulates proliferation and cellularity in the adventitia. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 3 caption a7 caption a8 PROLI/NO differentially affects cellularity throughout the arterial wall after balloon injury The carotid artery balloon injury model was performed in 10-week-old Sprague Dawley rats with or without periadventitial application of PROLI/NO (10 mg).

Techniques: Staining, Control

Journal: Free radical biology & medicine

Article Title: Long-Term Effect of PROLI/NO on the Proliferative and Phenotypic Kinetic Profile after Arterial Injury

doi: 10.1016/j.freeradbiomed.2015.11.027

Figure Lengend Snippet: The carotid artery balloon injury model was performed in 10-week-old Sprague Dawley rats with or without periadventitial application of PROLI/NO (10 mg). Carotids were harvested at different time points and sections were stained with an antibody to desmin. Quantification was performed by grading on a scale of 0–4 by a blinded investigator. A) Total grade sum throughout the arterial wall. B) Grade for the intima. C) Grade for the media. D) Grade for the adventitia. E) Representative arterial cross sections 2 weeks after balloon injury. #P<0.05 vs uninjured control, *P<0.05 vs. injury alone, N=7/treatment group.

Article Snippet: Whereas PROLI/NO inhibits proliferation and cellularity in the intima and media at all time points without having an effect on cell death, it initially inhibits but then stimulates proliferation and cellularity in the adventitia. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 3 caption a7 caption a8 PROLI/NO differentially affects cellularity throughout the arterial wall after balloon injury The carotid artery balloon injury model was performed in 10-week-old Sprague Dawley rats with or without periadventitial application of PROLI/NO (10 mg).

Techniques: Staining, Control

Journal: Free radical biology & medicine

Article Title: Long-Term Effect of PROLI/NO on the Proliferative and Phenotypic Kinetic Profile after Arterial Injury

doi: 10.1016/j.freeradbiomed.2015.11.027

Figure Lengend Snippet: The carotid artery balloon injury model was performed in 10-week-old Sprague Dawley rats with or without periadventitial application of PROLI/NO (10 mg). Carotid arteries were harvested at different time points and sections were stained with an antibody to NMMHC. Quantification was performed by grading on a scale of 0–4 by a blinded investigator. A) Total grade sum throughout the arterial wall. B) Grade for the intima. C) Grade for the media. D) Grade for the adventitia. E) Representative arterial cross sections 2 weeks after balloon injury. #P<0.05 vs. uninjured control, *P<0.05 vs. injury alone, N=7/treatment group.

Article Snippet: Whereas PROLI/NO inhibits proliferation and cellularity in the intima and media at all time points without having an effect on cell death, it initially inhibits but then stimulates proliferation and cellularity in the adventitia. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 3 caption a7 caption a8 PROLI/NO differentially affects cellularity throughout the arterial wall after balloon injury The carotid artery balloon injury model was performed in 10-week-old Sprague Dawley rats with or without periadventitial application of PROLI/NO (10 mg).

Techniques: Staining, Control

Journal: Free radical biology & medicine

Article Title: Long-Term Effect of PROLI/NO on the Proliferative and Phenotypic Kinetic Profile after Arterial Injury

doi: 10.1016/j.freeradbiomed.2015.11.027

Figure Lengend Snippet: The carotid artery balloon injury model was performed in 10-week-old Sprague Dawley rats with or without periadventitial application of PROLI/NO (10 mg). Carotids were harvested at different time points and sections were stained with an antibody against CD45. CD45+ cells were counted in four high power fields per section and averaged. A) Total cell count of CD45+ cells throughout the arterial wall. B) CD45+ cell count in the intima. C) CD45+ cell count in the media. D) CD45+ cell count in the adventitia. E) Representative arterial cross sections 2 weeks after balloon injury. The black arrow heads indicate representative CD45+ cells, and the blue arrow heads point to representative CD45-negative cells (only a few representative cells are labeled). #P<0.05 vs. uninjured control, *P<0.05 vs. injury alone, N=7/treatment group.

Article Snippet: Whereas PROLI/NO inhibits proliferation and cellularity in the intima and media at all time points without having an effect on cell death, it initially inhibits but then stimulates proliferation and cellularity in the adventitia. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 3 caption a7 caption a8 PROLI/NO differentially affects cellularity throughout the arterial wall after balloon injury The carotid artery balloon injury model was performed in 10-week-old Sprague Dawley rats with or without periadventitial application of PROLI/NO (10 mg).

Techniques: Staining, Cell Counting, Labeling, Control